ĭespite the popularity of the traditional WB as a key method, it also has its problems. This is normally performed by separating proteins using gel electrophoresis, transferring them to a membrane, and probing the target proteins with specific antibodies (Abs), followed by imaging of the Ab binding either via an enzymatic color reaction, a fluorescent label, or an enzyme-activated chemiluminescence signal. The key benefit of WB is the ability to identify specific target proteins from complex protein samples, such as tissue or cell lysates. ![]() ![]() Western blotting (WB) was invented over four decades ago and is still one of the most utilized analytical techniques in multiple different fields of research and diagnostics. Nevertheless, automation can be a good option to increase output and facilitate sensitive protein analyses. The downside of automation is the cost of devices and reagents. This is particularly beneficial for limited sample amounts. We found that a fully automated system can save time and importantly offer valuable sensitivity. We directly compared traditional Western blotting with two different automated systems, iBind™ Flex, which is a semi-automated system designed to perform the immunoblotting, and JESS Simple Western™, a fully automated and capillary-based system performing all steps downstream of sample preparation and loading, including imaging and image analysis. ![]() These include semi-automated techniques and fully automated devices that replicate all stages downstream of the sample preparation, including sample size separation, immunoblotting, imaging, and analysis. Consequently, devices with different degrees of automation have been developed. However, it can be time-consuming and suffer from a lack of reproducibility. Traditional Western blotting is one of the most used analytical techniques in biological research.
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